00:01To perform a urine colony count, take a sterile wire loop and pick 1 microliter of urine.
00:16Streak the urine down the middle of the plate and then streak from side to side to distribute the organisms
00:27evenly on the plate.
00:28You may then incubate the plates at 37 degrees Celsius for 24 to 48 hours.
00:39After the plates have incubated for 24 to 48 hours at 37 degrees Celsius, count each colony on the plate.
00:49Each colony represents one bacterium which forms a colony and is called a colony forming unit.
00:59To perform the Kirby biosensitivity test, take a sterile swab and pick a single colony.
01:18Inoculate a lawn on the Mueller-Hinton agar by spreading the bacteria evenly to cover the whole plate.
01:31You can spread the bacteria again in the perpendicular direction.
01:45Take your antibiotic discs and place them in the center of the inoculated Mueller-Hinton agar.
01:53You may use a sterile swab to gently tap the disc to stick it on the agar.
02:00Incubate the plates upside down at 37 degrees Celsius for another 24 to 48 hours.
02:10After the bacteria have grown, you can see a clearing around the antibiotic discs called the zones of inhibition.
02:19The bacteria are only susceptible to some kinds of antibiotics.
02:23The bacteria are not
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